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InvivoGen bhi broth
Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
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sterile brain heart infusion bhi broth  (Difco)
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Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Sterile Brain Heart Infusion Bhi Broth, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bhi broth  (Difco)
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Difco bhi broth
Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Bhi Broth, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain heart infusion bhi broth  (Difco)
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Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
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bhi criterion brain heart infusion bhi broth  (Bacto Laboratories)
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Bacto Laboratories bhi criterion brain heart infusion bhi broth
Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Bhi Criterion Brain Heart Infusion Bhi Broth, supplied by Bacto Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bhi criterion brain heart infusion bhi broth - by Bioz Stars, 2026-06
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bhi broth  (Condalab)
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Condalab bhi broth
Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Bhi Broth, supplied by Condalab, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain heart infusion bhi broth  (Beijing Land Bridge Technology Co Ltd)
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Beijing Land Bridge Technology Co Ltd brain heart infusion bhi broth
Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Brain Heart Infusion Bhi Broth, supplied by Beijing Land Bridge Technology Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain heart infusion bhi broth  (Liofilchem)
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Liofilchem brain heart infusion bhi broth
Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Brain Heart Infusion Bhi Broth, supplied by Liofilchem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brain heart infusion bhi broth  (Qingdao Hope Bio)
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Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Brain Heart Infusion Bhi Broth, supplied by Qingdao Hope Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sterile bhi broth  (Difco)
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Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume <t>BHI</t> was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 <t>µg/ml</t> <t>Pam3CSK4</t> was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.
Sterile Bhi Broth, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume BHI was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 µg/ml Pam3CSK4 was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.

Journal: Journal of Extracellular Vesicles

Article Title: Outer Membrane Vesicles Mediate the Secretion and Nuclear Trafficking of a Bacterial Nucleomodulin

doi: 10.1002/jev2.70286

Figure Lengend Snippet: Effects of EV‐associated Tipα on proinflammatory cytokine responses in host cells . (a–c) AGS and THP‐1 cells were incubated with UC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA ( tipA+) bacteria, corrected to 10 10 EV particles. As a positive control, rTipα (50 µg/ml) was added to cells. Detection of (a) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (b) IL‐8 produced by THP‐1 cells and (c) AGS cells (at 24 h post‐incubation). (d, e) THP‐1 cells were incubated with 1 × 10 10 /ml SEC EVs from either H. pylori SS1 WT, tipA mutant, or complemented tipA (tipA+) bacteria. As a negative control, an equivalent volume BHI was added to cells. Detection of (d) TNF produced by THP‐1 cells (at 6 h post‐incubation) and (e) IL‐8 by THP‐1 cells (at 24 h post‐incubation). (f) WT and Tlr2 −/− Tlr4 −/− iMacs were incubated with 1 × 10 10 /ml SEC or UC EVs. As a negative control, an equivalent volume of BHI was added to cells. As a positive control, 1 µg/ml Pam3CSK4 was added to cells. TNF production was detected at 6 h post‐incubation. Each data point corresponds to the mean of 3 technical replicates from one independent experiment and normalised relative to the median value for NS cells. Data were combined from (a–e) 3–4 and (f) 2–3 independent experiments. Histograms correspond to the means ± SEM. Statistical analyses were determined by either (a–e) one‐way ANOVA with Tukey's multiple comparisons or (f) two‐way ANOVA with multiple comparisons.

Article Snippet: As controls, cells we co‐cultured with either BHI broth alone (negative) or Pam3CSK4 (1 μg/ml, w/v; InvivoGen; positive).

Techniques: Incubation, Mutagenesis, Bacteria, Positive Control, Produced, Negative Control